Tabs Bands Ribbon & Preferences Text and Draw Tools Try clicking on the different tabs to see what types of actions are available.Ģ0 Within Layout Editor Graphs can be organized and re-formatted. Layout Editorġ9 Working in Layout Editor Similar to the Workspace, the Layout Editor has its own customizable Ribbon with Tabs and Bands to organize actions. Drag populations from a sample to Layout Editor. Type L, or click on the Layout Editor icon. Parent Child Siblingsġ8 The Layout Editor A tool for creating graphical reports. The subset population is a child of the parent sample. Custom Transformġ7 Gating Hierarchy When you create a gate on a sample, FlowJo shows you this gate (subset population) as a genealogical tree.
Click the Transformation icon to change the visual display. Explore the gating options and pick what works best for you.ġ6 Transforming Data Your data may initially look squished. Gating Tools Undo!! Gates can always be modified or removed, so don t be shy. Click on the Options Menu below the graph image and select graph Type from the dropdown menu.ġ4 Graph Display Options Try them all and pick what pleases you, or best represents your data.ġ5 Gating tools Are located at the top of a Graph Window. Options Graph Type Several different kind of plots are available to display flow data. (Workspace Tab à Add Keywords or Configure Tab à Edit Columns) Double click on a sample to open a Graph Window and add gates.ġ3 Graphs The Graph Window facilitates data visualization and gating. Additional information can be displayed as columns. Statistic and #Cells columns are displayed by default. Double click on an existing group to edit its properties.ġ2 Samples and Sample Analysis Displays the sample list and associated analysis of the currently selected group. Group Analysis displays all analysis within a group.ġ1 Creating and Editing Groups To create a new group type G, or click the Create Group Icon located in either the task bar at the top of the workspace, or within the Navigate band. Groups act like folders to organize your sample files and allow unified master gating and analysis. Press Ĩ Todays Demo Data Set: Phospho-Flow + Intracellular Cytokine Staining (PFICS) Polyclonal PFICS Assay: Thaw and rest cryopreserved human PBMC overnight No (NS) or with PMA+Ionomycin (PMA+I or PI) for 2 hours Stain for viability (AARD) and surface (CD3, CD4, CD8, CD38 and HLA- DR) No or PMA+I for 20 minutes Fix, permeabilize and stain for intracellular (phopho- ERK1/2, IFN- γ and Perforin)ĩ PFICS Stim Conditions 2 à 4 Thaw&Rest PBMC No S%m Stain Surface No S%m Fix&Perm Stain Intracellular NS+NS Total Time 0 min Thaw&Rest PBMC No S%m Stain Surface PMA+I Fix&Perm Stain Intracellular NS+PI 20 min Thaw&Rest PBMC PMA+I Stain Surface No S%m Fix&Perm Stain Intracellular PI+NS 120 min Thaw&Rest PBMC PMA+I Stain Surface PMA+I Fix&Perm Stain Intracellular PI+PI 140 min 5 donors X 4 = 20 experimental samples 1 donor with 7 FMOs X 2 = 14 FMO controls 12 Controlsġ0 Groups and Group Analysis The Group area lists all groups in the Workspace, # of samples in each group (Size), and the Role of that group (ex. Drag the icon for any Band into the ribbon to add that set of Actions to your selected Tab.ħ Importing Data Into FlowJo Three possible methods: 1. Bands group similar Actions together.Ħ Customizing Ribbons Click on the Ribbon icon to configure Click on Simple, Experienced or Advanced to change the number of Tabs displayed. Application Button Task Bar Tabs Bands Ribbon Configuration & Preferences Tabs group similar Bands together. Ribbon Tabs and Bands Groups and group analysis Samples and sample analysisĥ Ribbons, Tabs and Bands Ribbon organization allows easy visual navigation of workspace functions. Sophisticated tools allow generation of graphs and statistical reports, driving discovery of biological mechanisms.Ĥ The FlowJo v10 Workspace A graphical interface to organize your data. Uniformly analyze whole experiments encompassing many related samples.
1 Cytometry Data Analysis in FlowJo V10 Timothy Quinn Crawford, PhD Application Scientist FlowJo, LLCĢ Outline Part I What is FlowJo? Navigating the V10 Workspace Customizing Ribbons Demo Data Background Creating and Editing Groups Graphs, Gating and Ancestry The Layout Editor Batching and Exporting Graphics The Table Editorģ What is FlowJo? An integrated environment for viewing and analyzing flow cytometry data.
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